Effect of septal ablation on choline acetyltransferase in the paraventricular nucleus.

Article date: 1989/2/1

PubMed ID: 2706539

Journal name: Brain research bulletin (ISSN: 0361-9230)


To determine whether there is a cholinergic projection from the lateral septum to the paraventricular nucleus of the hypothalamus (PVH), a quantitative histochemical analysis of the effects of unilateral ablation (0.5 microliter of a 10 micrograms/ml solution of ibotenic acid) of the lateral septum (LSV) on the choline acetyltransferase (ChAT) activity of the PVH and surrounding perinuclear area was undertaken. Comparisons of the ChAT activity of the PVH region ipsilateral to the LSV lesion (lesioned side) with that of the PVH contralateral (nonlesioned side) to the ablated LSV showed a 20% reduction (p less than 0.05) of ChAT activity in 2 areas of the PVH on the lesioned side. When ChAT activity of LSV-ablated and intact control rats was compared, a 20% difference in activity (p less than 0.05) was noted in four areas on the nonlesioned side and a 35% difference (p less than 0.05) of ChAT activity was noted in five regions of the PVH on the lesioned side of LSV-ablated animals. Taken together these data suggest that the pathway from the LSV to the PVH contains a small, bilateral cholinergic component. However, the data also indicate that this is not the only cholinergic projection to the PVH.

Author List: Oorjitham E G, Godfrey D A, Ross C D, Dunn J D

Publication Types: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.

Substances mentioned in the article: Ibotenic Acid; Choline O-Acetyltransferase;

Mesh terms: Animals; Choline O-Acetyltransferase/metabolism; Cholinergic Fibers/cytology; Female; Ibotenic Acid; Paraventricular Hypothalamic Nucleus/cytology; Rats; Rats, Inbred Strains; Septal Nuclei/cytology;

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