Article date: 1989/9/25
PubMed ID: 2812533
Journal name: Neuroscience letters (ISSN: 0304-3940)
We examined the pattern of [3H]muscimol labeling in cat superior colliculus to determine if it matches that of [3H] gamma-aminobutyric acid ([3H]-GABA) labeling or GABA antibody immunoreactivity. Injections in the superficial layers labeled cell bodies in only the superficial layers. Of 204 labeled cells, 68% were located within the upper 200 microns of the superior colliculus, 31% within the deep superficial gray layer, and only 1% below that layer, a pattern similar to that seen with [3H]GABA labeling. By contrast, an injection in the deep layers of the colliculus resulted in cell labeling in both the superficial and deep layers, including 68% in the superficial gray layer, 24% in the optic layer, and 8% in the intermediate and deep gray layers. This pattern approximates that seen with GABA immunocytochemistry. We conclude that the pattern of accumulation of [3H]muscimol depends critically upon the location of the injection and reasonably matches the pattern of GABA immunoreactivity if the injection involves the deep layers of the colliculus.
Author List: Mize R R, White D A
Publication Types: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.
Substances mentioned in the article: Muscimol; gamma-Aminobutyric Acid;
Mesh terms: Animals; Autoradiography; Cats; Microscopy, Electron; Muscimol/metabolism; Neurons/metabolism; Superior Colliculi/cytology; gamma-Aminobutyric Acid/metabolism;