[3H]muscimol labels neurons in both the superficial and deep layers of cat superior colliculus.

Article date: 1989/9/25

PubMed ID: 2812533

Journal name: Neuroscience letters (ISSN: 0304-3940)


We examined the pattern of [3H]muscimol labeling in cat superior colliculus to determine if it matches that of [3H] gamma-aminobutyric acid ([3H]-GABA) labeling or GABA antibody immunoreactivity. Injections in the superficial layers labeled cell bodies in only the superficial layers. Of 204 labeled cells, 68% were located within the upper 200 microns of the superior colliculus, 31% within the deep superficial gray layer, and only 1% below that layer, a pattern similar to that seen with [3H]GABA labeling. By contrast, an injection in the deep layers of the colliculus resulted in cell labeling in both the superficial and deep layers, including 68% in the superficial gray layer, 24% in the optic layer, and 8% in the intermediate and deep gray layers. This pattern approximates that seen with GABA immunocytochemistry. We conclude that the pattern of accumulation of [3H]muscimol depends critically upon the location of the injection and reasonably matches the pattern of GABA immunoreactivity if the injection involves the deep layers of the colliculus.

This document is available from: http://directlinks.cc/files/muscimol/2812533.pdf

Author List: Mize R R, White D A

Publication Types: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.

Substances mentioned in the article: Muscimol; gamma-Aminobutyric Acid;

Mesh terms: Animals; Autoradiography; Cats; Microscopy, Electron; Muscimol/metabolism; Neurons/metabolism; Superior Colliculi/cytology; gamma-Aminobutyric Acid/metabolism;

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