Article date: 1988/2/9
PubMed ID: 2836212
Journal name: European journal of pharmacology (ISSN: 0014-2999)
Substances were found in purified fractions from pig brain that enhanced the specific binding of [3H]diazepam to membranes from rat brain in vitro. These substances were identified as oleic acid, arachidonic acid and docosahexenoic acid. Oleic acid (10(-5) - 10(-4)M) increased the affinity for agonists binding to the benzodiazepine receptor, whereas the binding of antagonists to this receptor was only slightly enhanced. The number of [3H]muscimol binding sites was increased, whereas binding of [3H]SR 95331, a GABA receptor antagonist, was unchanged. The effect of oleic acid was additional to the GABA-induced enhancement of [3H]diazepam binding.
Author List: Nielsen M, Witt M R, Thøgersen H
Publication Types: Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Arachidonic Acids; Fatty Acids, Unsaturated; Oleic Acids; Receptors, GABA-A; Docosahexaenoic Acids; Arachidonic Acid; Oleic Acid; Diazepam;
Mesh terms: Animals; Arachidonic Acid; Arachidonic Acids/isolation & purification; Brain Chemistry; Cerebral Cortex/drug effects; Diazepam/metabolism; Docosahexaenoic Acids/isolation & purification; Fatty Acids, Unsaturated/isolation & purification; In Vitro Techniques; Oleic Acid; Oleic Acids/isolation & purification; Rats; Receptors, GABA-A/drug effects; Swine;