gamma-Aminobutyric acid/benzodiazepine receptor protein carries binding sites for both ligands on both two major peptide subunits.

Article date: 1988/6/30

PubMed ID: 2839165

Journal name: Biochemical and biophysical research communications (ISSN: 0006-291X)


Affinity column-purified GABA-benzodiazepine receptor proteins from human, cow, and rat brain were photoaffinity labeled with both [3H]flunitrazepam and [3H]muscimol and examined by gel electrophoresis in sodium dodecyl sulfate. Using high receptor protein concentrations (1 microM), the benzodiazepine ligand [3H]flunitrazepam was incorporated covalently primarily into the expected 52 kiloDalton major subunit but also significantly into a second 57 kiloDalton peptide. Likewise the GABA ligand [3H]muscimol photolabeled primarily the 57 kiloDalton peptide but also to some extent the 52 kiloDalton peptide. This cross-labeling suggests strongly that both major subunits carry binding sites for both GABA and benzodiazepine.

This document is available from: http://directlinks.cc/files/muscimol/2839165.pdf

Author List: Bureau M, Olsen R W

Publication Types: Journal Article; Research Support, U.S. Gov't, P.H.S.

Substances mentioned in the article: Affinity Labels; Ligands; Macromolecular Substances; Receptors, GABA-A; Muscimol; Flunitrazepam;

Mesh terms: Affinity Labels/metabolism; Animals; Binding Sites; Cattle; Flunitrazepam/metabolism; Humans; Ligands/metabolism; Macromolecular Substances; Molecular Weight; Muscimol/metabolism; Photochemistry; Rats; Receptors, GABA-A/metabolism;

2839165.txt ยท Last modified: 2018/11/22 21:16 (external edit)