Article date: 1988/10/17
PubMed ID: 2849072
Journal name: Neuroscience letters (ISSN: 0304-3940)
Intracellular and voltage-clamp recordings were made from sensory neurons in bullfrog dorsal root ganglia (DRG). Bath-application of 5-hydroxytryptamine (5-HT, 10 microM to 1 mM) reversibly increased the amplitude of depolarizing responses to gamma-aminobutyric acid (GABA) and muscimol. 5-HT also increased the amplitude of chloride current activated by GABA. An analysis with dose-response curves revealed that 5-HT potentiated the maximum GABA current (Vmax), while it produced no significant change in the apparent dissociation constant (Km). It is suggested that 5-HT increases the sensitivity of the GABAA receptor, acting on an allosteric site for the receptor-ionophore complex.
Author List: Akasu T
Publication Types: Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Receptors, GABA-A; Muscimol; Serotonin; gamma-Aminobutyric Acid;
Mesh terms: Animals; Ganglia, Spinal/drug effects; In Vitro Techniques; Membrane Potentials/drug effects; Muscimol/pharmacology; Neurons, Afferent/drug effects; Rana catesbeiana/physiology; Receptors, GABA-A/drug effects; Serotonin/pharmacology; gamma-Aminobutyric Acid/pharmacology;