2859566

The excitation of frog motoneurones in vitro by the glutamate analogue, DI-alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA), and the effect of amino acid antagonists.

Article date: 1985/3/22

PubMed ID: 2859566

Journal name: Neuroscience letters (ISSN: 0304-3940)

ABSTRACT

Intracellular recordings were made from motoneurones of the frog spinal cord in vitro and the excitatory effects of the glutamate analogue DL-alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) examined. AMPA (25-100 microM) depolarized motoneurones with an increase in input conductance and an increase in spontaneous cell firing. The time-course of the AMPA response was prolonged compared to L-glutamate. D-Aminophosphonovalerate (1 microM) and D-aminoadipate (100 microM) did not antagonize AMPA-induced excitations while depressing L-glutamate responses. Glutamate diethylester up to 1 mM was not found to be a useful amino acid antagonist since it did not significantly reduce the depolarizations to quisqualate, L-glutamate and AMPA. The results are compatible with an action of AMPA on receptors distinct from those to N-methyl-D-aspartate and shared by quisqualate.

Author List: King A E, Nistri A, Rovira C

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Amino Acids, Dicarboxylic; Glutamates; Oxadiazoles; Oxazoles; Receptors, AMPA; Receptors, Drug; glutamic acid diethyl ester; 2-Aminoadipic Acid; Ibotenic Acid; Glutamic Acid; 2-Amino-5-phosphonovalerate; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Quisqualic Acid; Valine;

Mesh terms: 2-Amino-5-phosphonovalerate; 2-Aminoadipic Acid/pharmacology; Amino Acids, Dicarboxylic/pharmacology; Animals; Glutamates/pharmacology; Glutamic Acid; Ibotenic Acid/analogs & derivatives; Membrane Potentials/drug effects; Motor Neurons/drug effects; Oxadiazoles/pharmacology; Oxazoles/pharmacology; Quisqualic Acid; Rana temporaria; Receptors, AMPA; Receptors, Drug/drug effects; Spinal Cord/drug effects; Synapses/drug effects; Synaptic Transmission/drug effects; Valine/analogs & derivatives; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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