L-[3H]Glutamate binds to kainate-, NMDA- and AMPA-sensitive binding sites: an autoradiographic analysis.

Article date: 1985/8/12

PubMed ID: 2862960

Journal name: Brain research (ISSN: 0006-8993)


The anatomical distribution of L-[3H]glutamate binding sites was determined in the presence of various glutamate analogues using quantitative autoradiography. The binding of L-[3H]glutamate is accounted for by the presence of 3 distinct binding sites when measured in the absence of Ca2+, Cl- and Na+ ions. The anatomical distribution and pharmacological specificity of these binding sites correspond to that reported for the 3 excitatory amino acid binding sites selectively labelled by D-[3H]2-amino-5-phosphonopentanoate (D-[3H]AP5), [3H]kainate ([3H]KA) and [3H] alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ([3H]AMPA) which are thought to be selective ligands for the N-methyl-D-aspartate (NMDA), KA and quisqualate (QA) receptors, respectively.

This document is available from: http://directlinks.cc/files/muscimol/2862960.pdf

Author List: Monaghan D T, Yao D, Cotman C W

Publication Types: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.

Substances mentioned in the article: Glutamates; Pyrrolidines; Receptors, Glutamate; Receptors, Neurotransmitter; Ibotenic Acid; Aspartic Acid; Glutamic Acid; N-Methylaspartate; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Kainic Acid;

Mesh terms: Animals; Aspartic Acid/analogs & derivatives; Autoradiography; Binding Sites/drug effects; Brain/anatomy & histology; Brain Chemistry/drug effects; Glutamates/metabolism; Glutamic Acid; Ibotenic Acid/analogs & derivatives; In Vitro Techniques; Kainic Acid/metabolism; N-Methylaspartate; Pyrrolidines/metabolism; Rats; Rats, Inbred Strains; Receptors, Glutamate; Receptors, Neurotransmitter/analysis; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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