2897429

Characterisation of Na+-independent L-[3H]glutamate binding sites in human temporal cortex.

Article date: 1988/6/1

PubMed ID: 2897429

Journal name: Journal of neurochemistry (ISSN: 0022-3042)

ABSTRACT

The binding of L-[3H]glutamate to membranes from human temporal cortex was studied in the absence of Na+, Ca2+, and Cl- ions. Pharmacological characterisation revealed that approximately 35% of specific binding at 50 nM L-[3H]glutamate was sensitive to a combination of kainate and alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid. The remaining approximately 65% of specific binding was to a single population of sites with a KD of 844 nM and a Bmax of 0.92 pmol/mg protein. The pharmacological characteristics were consistent with an interaction at the N-methyl-D-aspartate subclass of excitatory amino acid receptor. The inclusion of Cl- ions revealed additional glutamate binding; this was sensitive to quisqualate and DL-2-amino-4-phosphonobutyrate, but not to kainate, DL-2-amino-7-phosphonoheptanoate, or alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid.

Author List: Cowburn R F, Hardy J A, Roberts P J

Publication Types: Journal Article

Substances mentioned in the article: Amino Acids; Aminobutyrates; Chlorides; Glutamates; Oxadiazoles; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter; Ibotenic Acid; Glutamic Acid; 2-Amino-5-phosphonovalerate; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Quisqualic Acid; Sodium; 2-amino-4-phosphonobutyric acid; 2-amino-7-phosphonoheptanoic acid; Kainic Acid;

Mesh terms: 2-Amino-5-phosphonovalerate/analogs & derivatives; Aged; Amino Acids/pharmacology; Aminobutyrates/pharmacology; Cell Membrane/metabolism; Chlorides/pharmacology; Glutamates/metabolism; Glutamic Acid; Humans; Ibotenic Acid/analogs & derivatives; Kainic Acid/pharmacology; Male; Middle Aged; Oxadiazoles/pharmacology; Quisqualic Acid; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter/metabolism; Sodium/pharmacology; Temporal Lobe/drug effects; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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