Article date: 1988/8/1
PubMed ID: 2899131
Journal name: Journal of neurochemistry (ISSN: 0022-3042)
Binding of [3H](R,S)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ([3H]AMPA) to quisqualate receptors in the presence of SCN- ions produced curvilinear Scatchard plots. Kinetic investigations of [3H]AMPA binding showed that the curvilinearity cannot be explained by assuming binding to two separate binding sites or by considering it due to cooperative interaction. A more likely explanation is that the quisqualate receptors exist in two states, one with high and one with low affinity for [3H]AMPA. Chaotropic ions change the relaxation constant between the two states.
Author List: Honoré T, Drejer J
Publication Types: Journal Article
Substances mentioned in the article: Cyanates; Cyanides; Receptors, AMPA; Receptors, Drug; Ibotenic Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;
Mesh terms: Animals; Cerebral Cortex/drug effects; Cyanates/pharmacology; Cyanides/pharmacology; Ibotenic Acid/analogs & derivatives; Kinetics; Membranes/drug effects; Protein Conformation/drug effects; Rats; Receptors, AMPA; Receptors, Drug/drug effects; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;