Article date: 1985/2/4
PubMed ID: 2982067
Journal name: Life sciences (ISSN: 0024-3205)
GABA, THIP and muscimol enhance spontaneous and inhibit electrically induced release of tritium labelled compounds from rat striatal slices which have been pre-labelled with 3H-choline. Baclofen is inactive in this model. Muscimol can inhibit electrically induced release of tritiated material by approximately 75% with half maximal effects at 2 microM. The response to muscimol can be blocked by the GABA antagonists bicuculline methobromide, picrotoxin, anisatin, R 5135 and CPTBO (cyclopentylbicyclophosphate). Drugs which act on the benzodiazepine receptor (BR) require the presence of muscimol to be effective and they modulate the effects of muscimol in a bidirectional manner. Thus BR agonists enhance and inverse BR agonists attenuate the inhibitory effects of muscimol on electrically induced release. Ro15-1788, a BR antagonist, does not modulate the inhibitory effects of muscimol but antagonizes the actions of clonazepam, a BR agonist, and of DMCM, an inverse BR agonist. These results demonstrate that a GABA/benzodiazepine receptor complex can modulate acetylcholine release from rat striatal slices in vitro.
Author List: Supavilai P, Karobath M
Publication Types: Journal Article
Substances mentioned in the article: Benzodiazepinones; Isoxazoles; Receptors, GABA-A; Muscimol; Flumazenil; gamma-Aminobutyric Acid; Clonazepam; Atropine; Baclofen; Haloperidol; gaboxadol; Acetylcholine;
Mesh terms: Acetylcholine/metabolism; Animals; Atropine/pharmacology; Baclofen/pharmacology; Benzodiazepinones/pharmacology; Clonazepam/pharmacology; Corpus Striatum/metabolism; Electric Stimulation; Flumazenil; Haloperidol/pharmacology; In Vitro Techniques; Isoxazoles/pharmacology; Muscimol/pharmacology; Rats; Rats, Inbred Strains; Receptors, GABA-A/metabolism; gamma-Aminobutyric Acid/pharmacology;