gamma-Aminobutyric acidA (GABAA) receptors modulate [3H]GABA release from isolated neuronal growth cones in the rat.

Article date: 1985/4/19

PubMed ID: 2989732

Journal name: Neuroscience letters (ISSN: 0304-3940)


Potassium-induced release of gamma-[3H]aminobutyric acid [( 3H]GABA) from a growth cone-enriched fraction isolated from neonatal rat forebrain was inhibited by the GABA mimetic muscimol in a dose-dependent manner (IC50 15 nM). The GABA antagonist bicuculline completely reversed the effect of muscimol. Bicuculline alone slightly potentiated the K+-induced release of [3H]GABA. Baclofen, a proposed selective agonist for a bicuculline-insensitive GABAB receptor, was found to cause only a slight reduction in the K+-induced release of [3H]GABA. These results are compatible with the presence of a negative feedback mechanism mediated by GABAA receptors for controlling [3H]GABA release from growth cones of the developing rat forebrain.

This document is available from: http://directlinks.cc/files/muscimol/2989732.pdf

Author List: Lockerbie R O, Gordon-Weeks P R

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Azides; Benzamides; Receptors, GABA-A; Tritium; Muscimol; bicuculline methiodide; gamma-Aminobutyric Acid; 4-azido-5-iodoclebopride; Baclofen; Potassium; Bicuculline;

Mesh terms: Animals; Animals, Newborn/metabolism; Axons/metabolism; Azides/pharmacology; Baclofen/pharmacology; Benzamides/pharmacology; Bicuculline/analogs & derivatives; Brain/metabolism; Dendrites/drug effects; Dose-Response Relationship, Drug; Homeostasis; Muscimol/pharmacology; Potassium/pharmacology; Rats; Receptors, GABA-A/physiology; Stimulation, Chemical; Subcellular Fractions/metabolism; Tritium; gamma-Aminobutyric Acid/metabolism;

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