2996303

Methylene blue inhibits GABA

Article date: 2017/4/10

PubMed ID: 2996303

Journal name: Neuropharmacology (ISSN: 1873-7064)

DOI: 10.1016/j.neuropharm.2017.04.002

ABSTRACT

Methylene blue (MB) is commonly used in diagnostic procedures and is also used to treat various medical conditions. Neurological effects of MB have been reported in clinical observations and experimental studies. Thus the modulation of GABA site is unlikely. MB-induced inhibition of GABA response was competitive with respect to GABA. Furthermore, mutation of α1 F64 to A and β2 Y205 to F in the extracellular N-terminus, both residues which are known to comprise GABA binding pocket, remarkably diminished MB inhibition of GABA currents. These data suggest that MB inhibits GABA

Author List: Chen Zhenglan, Liu Ran, Yang Shao-Hua, Dillon Glenn H, Huang Renqi

Publication Types: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Intramural; Research Support, N.I.H., Extramural

Article Date: 2017/04/05

Substances mentioned in the article: Enzyme Inhibitors; Receptors, GABA-A; Sodium Channel Blockers; Tetrodotoxin; gamma-Aminobutyric Acid; Methylene Blue;

Mesh terms: Animals; Animals, Newborn; Binding Sites/drug effects; Enzyme Inhibitors/pharmacology; Female; Gene Expression Regulation/drug effects; HEK293 Cells; Hippocampus/cytology; Humans; In Vitro Techniques; Inhibitory Postsynaptic Potentials/drug effects; Male; Methylene Blue/pharmacology; Mice; Mice, Inbred C57BL; Neurons/drug effects; Protein Binding/drug effects; Rats; Receptors, GABA-A/genetics; Sodium Channel Blockers/pharmacology; Tetrodotoxin/pharmacology; gamma-Aminobutyric Acid/metabolism;

Citations: - 9426470

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