Article date: 1985/10/24
PubMed ID: 3001591
Journal name: Neuroscience letters (ISSN: 0304-3940)
The purified gamma-aminobutyric acid-benzodiazepine receptor complex from bovine cerebral cortex has been reconstituted into phospholipid vesicles by a cholate dialysis procedure. The reconstituted receptor bound [3H]flunitrazepam at a single class of sites with dissociation constant Kd = 2.9 +/- 0.3 nM, an increase in affinity to the membrane level from the 4-fold weakening found in detergent solution. It also bound [3H]muscimol with a Kd for the high-affinity sites of approximately 50 nM. [35S]tert.-Butyl-bicyclophosphorothionate, for which there is evidence in membranes for binding to a channel gating site on this receptor, showed similar binding to the reconstituted receptor.
Author List: Sigel E, Mamalaki C, Barnard E A
Publication Types: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Liposomes; Phosphatidylcholines; Phosphatidylethanolamines; Phosphatidylserines; Phospholipids; Receptors, GABA-A; asolectin; Cholesterol;
Mesh terms: Animals; Cattle; Cerebral Cortex/analysis; Cholesterol; Liposomes; Phosphatidylcholines; Phosphatidylethanolamines; Phosphatidylserines; Phospholipids; Receptors, GABA-A/isolation & purification;