Article date: 1986/1/1
PubMed ID: 3012606
Journal name: Pharmacology (ISSN: 0031-7012)
The distribution of 3H-muscimol within guinea pig ovary was studied using combined radioreceptor binding studies and histoautoradiographic technique performed on frozen sections of ovary mounted on microscope slides. 3H-muscimol was bound by sections of ovary in a manner consistent with the existence of specific GABA receptors. The binding was reversible, saturable, of high affinity (KD was 38 +/- 6 nmol/l and Bmax was about 378 +/- 61 fmol/mg protein, and inhibited by GABA receptor interfering drugs. 3H-muscimol binding sites were found in the blood vessel wall, in the follicle and in the oocytes. The number of oocyte and follicular receptors gradually decreases during the development of ovarian follicles.
Author List: Amenta F, Erdo S L, Mione M C, Napoleone P
Publication Types: Journal Article
Substances mentioned in the article: Receptors, GABA-A; Muscimol; Bicuculline;
Mesh terms: Animals; Autoradiography; Bicuculline/pharmacology; Binding Sites; Female; Guinea Pigs; Histocytochemistry; In Vitro Techniques; Muscimol/metabolism; Ovary/metabolism; Receptors, GABA-A/metabolism;