Article date: 1986/8/14
PubMed ID: 3019340
Journal name: Biochemical and biophysical research communications (ISSN: 0006-291X)
Affinity column-purified GABA-benzodiazepine receptor protein from bovine brain was photoaffinity labeled with both [3H]flunitrazepam and [3H]muscimol. Gel electrophoresis in sodium dodecyl sulfate revealed that the benzodiazepine binding site labeled with [3H]flunitrazepam was primarily associated with a major peptide subunit revealed by protein staining with Mr = 52 kiloDaltons, with minor labeling of a second peptide of Mr = 57 kiloDaltons, corresponding to a second major stained band. Covalent incorporation of [3H]muscimol was limited to the 57 kiloDalton band, with no labeling of the 52 kiloDalton peptide, showing that the GABA binding site is carried by a subunit distinct from that carrying the benzodiazepine binding site.
Author List: Deng L, Ransom R W, Olsen R W
Publication Types: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
Substances mentioned in the article: Affinity Labels; Receptors, GABA-A; Benzodiazepines; Muscimol; Flunitrazepam;
Mesh terms: Affinity Labels; Animals; Benzodiazepines/metabolism; Binding Sites; Cattle; Cerebral Cortex; Flunitrazepam/metabolism; In Vitro Techniques; Muscimol/metabolism; Photochemistry; Receptors, GABA-A/metabolism; Time Factors; Ultraviolet Rays;