The enhancement of diazepam and muscimol binding by pentobarbital and (+)-etomidate: size of the molecular arrangement estimated by electron irradiation inactivation of rat cortex.

Article date: 1986/9/25

PubMed ID: 3022191

Journal name: Neuroscience letters (ISSN: 0304-3940)

ABSTRACT

Synaptosomal membranes were prepared from frozen rat cortices irradiated by 10 MeV electrons and the enhancement of [3H]diazepam and [3H]muscimol binding by pentobarbital (PB) and (+)-etomidate was studied. The target sizes of the corresponding parts of the receptor complex were estimated from the decrease in the enhancement as a function of irradiation dose. Different radiation inactivation constants suggest different regulatory units for the enhancement by PB and (+)-etomidate. Target sizes for PB and (+)-etomidate enhancement of [3H]diazepam binding were 127 +/- 14 and 360 + 124 kDa, respectively.

Author List: Maksay G, Nielsen M, Simonyi M

Publication Types: Comparative Study; Journal Article

Substances mentioned in the article: Receptors, GABA-A; Muscimol; gamma-Aminobutyric Acid; Pentobarbital; Diazepam; Etomidate;

Mesh terms: Animals; Cerebral Cortex/drug effects; Diazepam/metabolism; Etomidate/pharmacology; In Vitro Techniques; Molecular Weight; Muscimol/metabolism; Pentobarbital/pharmacology; Rats; Receptors, GABA-A/drug effects; gamma-Aminobutyric Acid/metabolism;