GABA enhances acetylcholine release from hippocampal nerve endings through a mechanism blocked by a GABA uptake inhibitor.

Article date: 1986/10/20

PubMed ID: 3022197

Journal name: Neuroscience letters (ISSN: 0304-3940)


The effect of gamma-aminobutyric acid (GABA) on the release of [3H]acetylcholine [( 3H]ACh) was investigated using superfused rat hippocampal synaptosomes. GABA enhanced the basal efflux of [3H]ACh. The effect of GABA was bicuculline-insensitive. Muscimol, (+/-)-baclofen or (-)-baclofen did not increase [3H]ACh release. The effect of GABA was counteracted by SK&F 89976 A (N-(4,4-diphenyl-3-butenyl)-nipecotic acid), a GABA uptake inhibitor. One possible interpretation of the results is that a GABA transport system is present on cholinergic terminals, suggesting that GABA and ACh may coexist in some rat hippocampus nerve endings. Another possibility is that the effect of GABA is mediated by a novel subtype of GABA receptor sensitive to SK&F 89976 A.

This document is available from: http://directlinks.cc/files/muscimol/3022197.pdf

Author List: Bonanno G, Raiteri M

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: GABA Antagonists; Nipecotic Acids; Receptors, GABA-A; gamma-Aminobutyric Acid; N-(4,4-diphenyl-3-butenyl)nipecotic acid; Acetylcholine; Bicuculline;

Mesh terms: Acetylcholine/secretion; Animals; Bicuculline/pharmacology; GABA Antagonists; Hippocampus/drug effects; Male; Nerve Endings/metabolism; Nipecotic Acids/pharmacology; Rats; Receptors, GABA-A/drug effects; Synaptosomes/metabolism; gamma-Aminobutyric Acid/pharmacology;

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