Article date: 1986/1/1
PubMed ID: 3022561
Journal name: Acta neurobiologiae experimentalis (ISSN: 0065-1400)
The distribution of binding sites for [3H] muscimol (which binds to GABA, receptor) and [3H] QNB (which binds to muscarinic cholinergic receptor) was studied in visual areas of 5 week-old kittens using contact film autoradiography after incubation in vitro of brain sections. A distinct laminar localization of [3H] muscimol receptor sites was observed in young animals with the highest density in layers II/ III and IV of all cortical areas that were investigated. For [3H] QNB binding a laminar pattern of distribution was also found with labeling in layer V exceeding that in adjacent layers. A small intracortical regional variability was noted, for both of the investigated neuroreceptors. [3H] muscimol labeling of the lateral geniculate nucleus and superior colliculus was weaker in comparison to that found in the cortex. The superficial layers of the superior colliculus revealed a high density of [3H] muscimol and [3H] QNB binding sites. The laminar and regional variations in the distribution of [3H] muscimol and [3H] QNB binding sites observed in young kittens, may be related to the possible role of GABAergic and muscarinic receptors in regulation of plastic changes that occur after visual deprivation.
Author List: Skangiel-Kramska J, Cymerman U, Kossut M
Publication Types: Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Receptors, GABA-A; Receptors, Muscarinic; Tritium; Muscimol; Quinuclidinyl Benzilate;
Mesh terms: Animals; Autoradiography; Brain/metabolism; Cats; Muscimol/metabolism; Quinuclidinyl Benzilate/metabolism; Receptors, GABA-A/metabolism; Receptors, Muscarinic/metabolism; Tritium; Visual Pathways/metabolism;