Article date: 1987/5/12
PubMed ID: 3038260
Journal name: Brain research (ISSN: 0006-8993)
The effect of gamma-aminobutyric acid (GABA)receptor stimulation on norepinephrine (NE)-induced metabolism of polyphosphoinositides (PIPs) was studied in rat hippocampal slices. Inositol phosphates (IPs), PIPs, and phosphatidic acid were measured. NE induced formation of IPs and phosphatidic acid in a dose-dependent manner with an EC50 of 4.5 microM. GABA, 3-aminopropanesulphonic acid (3APS) and muscimol did not affect PIPs breakdown, but they strongly increased (greater than 100%) PIPs metabolism induced by 1 microM NE. Their action was antagonized by bicuculline (10 microM). We discuss the implications of these findings in hippocampal neurotransmission.
Author List: Corradetti R, Ruggiero M, Chiarugi V P, Pepeu G
Publication Types: Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Inositol Phosphates; Phosphatidic Acids; Phosphatidylinositol Phosphates; Phosphatidylinositols; Receptors, GABA-A; Taurine; Muscimol; gamma-Aminobutyric Acid; tramiprosate; Norepinephrine; Bicuculline;
Mesh terms: Animals; Bicuculline/pharmacology; Hippocampus/drug effects; In Vitro Techniques; Inositol Phosphates/metabolism; Male; Muscimol/pharmacology; Norepinephrine/pharmacology; Phosphatidic Acids/metabolism; Phosphatidylinositol Phosphates; Phosphatidylinositols/metabolism; Rats; Rats, Inbred Strains; Receptors, GABA-A/drug effects; Taurine/analogs & derivatives; gamma-Aminobutyric Acid/pharmacology;