316440

Antibodies directed against a nonapeptide sequence of the gamma-aminobutyrate (GABA)/benzodiazepine receptor alpha-subunit. Detection of a distinct alpha-like subunit in pig cerebral cortex but not cerebellum.

Article date: 1988/11/15

PubMed ID: 316440

Journal name: The Biochemical journal (ISSN: 0264-6021)

ABSTRACT

A synthetic peptide, corresponding to amino acid residues 101-109 of the bovine gamma-aminobutyrate/benzodiazepine receptor alpha-subunit, was used to raise a polyclonal antiserum. The reactivity of this antiserum towards polypeptides of both bovine and pig receptor preparations was established by immunoprecipitation and immunoblotting. Anti-peptide antibodies recognized the alpha-subunit (51 kDa) of receptor prepared from pig cerebellum or cerebral cortex. However, a polypeptide of 57 kDa was additionally recognized in cortical, but not cerebellar, preparations. This alpha-like polypeptide appeared larger than the band of polypeptides labelled irreversibly with [3H]muscimol (beta-subunit, 55-57 kDa) and corresponds to a polypeptide detected only in cortex after silver-staining or irreversible labelling with [3H]flunitrazepam. These results support the idea that the distinct regional patterns of polypeptides labelled irreversibly with [3H]flunitrazepam reflect the existence of heterologous distributions of distinct alpha-like subunits.

Author List: Kirkness E F, Turner A J

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Affinity Labels; Peptides; Receptors, GABA-A; Muscimol; Flunitrazepam;

Mesh terms: Affinity Labels; Animals; Brain Chemistry; Cerebellum/analysis; Cerebral Cortex/analysis; Chemical Precipitation; Flunitrazepam; Immunoblotting; Muscimol; Peptides/immunology; Receptors, GABA-A; Swine;

Citations: - Purification and partial characterization of 4-aminobutyrate:2-oxoglutarate aminotransferase from sheep brain and locust ganglia.

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