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455057 [2018/11/20 14:26] (current)
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 +====== Spontaneous release of endogenous aspartate and glutamate from rat striatal slices is increased following destruction of local neurons by ibotenic acid.======
 +
 +** Article date:** 1988/5/1
 +~~META:date created = 1988-05-01~~
 +
 +** PubMed ID: 455057 **
 +
 +** Journal name: Neurochemical research (ISSN: 0364-3190) **
 +
 +** ABSTRACT **
 +
 +We sought to determine in rat striatum whether the release of neurotransmitter amino acids aspartate (Asp), glutamate (Glu) and gamma-aminobutyric acid (GABA) were affected by local neurons. To do so, unilateral microinjections of ibotenic acid, and excitotoxin that destroys local neurons without affecting fibers of passage, were made into the striatum. Release of endogenous amino acids from lesioned and intact striatal slices were measured by HPLC one week later. The effectiveness and specificity of the lesion were confirmed by measuring the enzyme activity associated with extrinsic dopamine neurons (tyrosine hydroxylase;​ 111 +/- 14%), intrinsic GABA neurons (glutamic acid decarboxylase;​ 19 +/- 7%) and intrinsic acetylcholine neurons (choline acetyltranferase;​ 37 +/- 10%). Destruction of local striatal neurons markedly attenuated the release of GABA (41 +/- 12% of control) elicited by depolarization with K+ (35 mM), but did not significantly reduced the K+-evoked release of Asp (80 +/- 17%) and Glu (92 +/- 8%). However, spontaneous release of Asp and Glu was significantly greater than that observed in unlesioned tissue (159 +/- 18% and 209 +/- 27%, respectively),​ while the spontaneous release of GABA was not significantly reduced (75 +/- 43%). Although release of the neurotransmitter amino acids Asp, Glu and GABA were affected by the lesion, the release of the non-neurotransmitter amino acid tyrosine was unaffected. These data are consistent with the hypotheses that: 1) the predominant source of releasable stores of endogenous Asp and Glu in the striatum arises from extinsic neurons, and 2) that the spontaneous release of Asp and Glu from axon terminals in the striatum may be regulated, at least in part, by local inhibitory neurons.
 +
 +===== ===== 
 +
 +Author List: Arnerić S P, Woo J I, Meeley M P, Reis D J
 +
 +Publication Types: Journal Article
 +
 +Substances mentioned in the article: Amino Acids; Glutamates; Oxazoles; Ibotenic Acid; Aspartic Acid; Glutamic Acid; gamma-Aminobutyric Acid; 
 +
 +Mesh terms: Amino Acids/​metabolism;​ Animals; Aspartic Acid/​metabolism;​ Corpus Striatum/​cytology;​ Glutamates/​metabolism;​ Glutamic Acid; Ibotenic Acid; In Vitro Techniques; Neurons/​physiology;​ Oxazoles; Rats; gamma-Aminobutyric Acid/​metabolism; ​
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 +Citations: ​  - //​[[6109000]]//​
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 +{{tag> Amino_Acids Ibotenic_Acid Glutamates gamma-Aminobutyric_Acid Oxazoles Glutamic_Acid Aspartic_Acid }}
 +
 +{{keywords>​ Amino Acids, Ibotenic Acid, Glutamates, gamma-Aminobutyric Acid, Oxazoles, Glutamic Acid, Aspartic Acid}}
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455057.txt · Last modified: 2018/11/20 14:26 (external edit)