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784912 [2018/11/20 14:26] (current)
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 +====== Non-NMDA excitatory amino acid receptors in a subcellular fraction enriched in cerebellar glomeruli.======
 +
 +** Article date:** 1991/4/1
 +~~META:date created = 1991-04-01~~
 +
 +** PubMed ID: 784912 **
 +
 +** Journal name: Neurochemical research (ISSN: 0364-3190) **
 +
 +** ABSTRACT **
 +
 +In the internal granular layer of the cerebellar cortex the polysynaptic complexes called glomeruli consist mainly of homogeneous populations of glutamatergic and GABAergic synapses, both located on granule cell dendrites. A subcellular fraction enriched in glomeruli was prepared from rat cerebellum, and the distribution of the different types of NMDA and non-NMDA glutamate binding sites was studied in the membranes derived from this fraction (fraction G) as compared to that in the membranes prepared from a total cerebellar homogenate (fraction T). Cl-/Ca2+ independent [3H]glutamate binding sites were not abundant and could be reliably measured only in fraction G. Cl- dependent/​Ca2+ activated [3H]glutamate binding sites were more abundant and exhibited a single KD in both fractions G and T. Quisqualate,​ NMDA, kainate, L-AP4 and trans-ACPD inhibited [3H]glutamate binding to different extents in the two membrane fractions. Quisqualate sensitive sites were predominant in all cases but more abundant in fraction T than in fraction G. An opposite distribution was observed for the NMDA sensitive binding sites while kainate sensitive binding sites were scarce everywhere. Trans-ACPD, a ligand presumed selective for metabotropic glutamate binding sites, displaced [3H]glutamate from fraction T but nor from fraction G, suggesting the absence of these sites from glomeruli. Similarly, no L-AP4 sensitive sites were present in fraction G while they were abundant in fraction T. Binding sites associated with ionotropic receptors of the quisqualate type were determined by measuring [3H]AMPA binding. The density of the high affinity [3H]AMPA binding sites in fraction T was twice as high as in fraction G, indicating that these sites are abundant in structures other than glomeruli.(ABSTRACT TRUNCATED AT 250 WORDS)
 +
 +===== ===== 
 +
 +Author List: Viennot F, de Barry J, Gombos G
 +
 +Publication Types: Journal Article
 +
 +Substances mentioned in the article: Glutamates; Receptors, AMPA; Receptors, Glutamate; Receptors, Kainic Acid; Receptors, Neurotransmitter;​ Ibotenic Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Kainic Acid; 
 +
 +Mesh terms: Animals; Binding, Competitive;​ Cells, Cultured; Cerebellum/​metabolism;​ Female; Glutamates/​metabolism;​ Ibotenic Acid/​analogs & derivatives;​ Kainic Acid/​metabolism;​ Kinetics; Male; Rats; Rats, Inbred Strains; Receptors, AMPA; Receptors, Glutamate; Receptors, Kainic Acid; Receptors, Neurotransmitter/​metabolism;​ alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; 
 +
 +Citations: ​  - //​[[6121375]]//​
 +  - //​[[3014386]]//​
 +  - //​[[20501304]]//​
 +  - //​[[1966569]]//​
 +  - //​[[2983842]]//​
 +  - //​[[2579123]]//​
 +  - //​[[5921132]]//​
 +  - //​[[1967632]]//​
 +  - //​[[4131274]]//​
 +  - //​[[2155495]]//​
 +  - //​[[1089552]]//​
 +  - //​[[6139153]]//​
 +  - //​[[1092812]]//​
 +  - //​[[4363490]]//​
 +  - //​[[6384832]]//​
 +  - //​[[2890112]]//​
 +  - //​[[492142]]//​
 +  - //​[[6151108]]//​
 +  - //​[[6145511]]//​
 +  - //​[[6466990]]//​
 +  - //​[[6254391]]//​
 +  - //​[[1969937]]//​
 +  - //​[[4027690]]//​
 +  - //​[[3031086]]//​
 +  - //​[[7205272]]//​
 +  - //​[[1973276]]//​
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 +  - //​[[14907713]]//​
 +  - //​[[6090209]]//​
 +  - //​[[1115992]]//​
 +  - //​[[2414684]]//​
 +  - //​[[784912]]//​
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 +{{tag> Receptors,​_Neurotransmitter Receptors,​_Glutamate Receptors,​_AMPA Glutamates Receptors,​_Kainic_Acid alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic_Acid Kainic_Acid Ibotenic_Acid }}
 +
 +{{keywords>​ Receptors, Neurotransmitter,​ Receptors, Glutamate, Receptors, AMPA, Glutamates, Receptors, Kainic Acid, alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid, Kainic Acid, Ibotenic Acid}}
 +
  
784912.txt · Last modified: 2018/11/20 14:26 (external edit)